How to Build Plasmid Engineering Workbench with Circular Mapping, Restriction Analysis, Virtual Gels, and Primer Design

In this tutorial, we build a Google Colab-native plasmid workbench that recreates the core ideas of SpliceCraft inside an interactive notebook environment. Instead of relying on a terminal-based TUI, we use Biopython, NumPy, and Matplotlib to load plasmid records, normalize annotated genomic features, render circular and linear plasmid maps, compute sequence statistics, analyze restriction enzyme…